Materials used to develop the Kit are RNA samples extracted from nCoV that cause disease in Vietnamese patients, provided by the National Institute of Hygiene and Epidemiology.
The Kit (biological kit) developed by the Institute of Biotechnology (Vietnam Academy of Science and Technology) has been announced by the research team on the afternoon of March 3, based on realtime molecular RT-PCR biotechnology (recommended by the WHO in the manufacture of the Test Kit).

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Biological kit was developed by the Institute of Biotechnology. Photo: Viet Linh.

Assoc.Prof.Dr. Dong Van Quyen, Deputy Director of the Institute of Biotechnology, Head of the research team said, Kit developed on the basis of primers and probes designed and adapted based on important genes and gene regions of SARS-Cov-2 isolated in Vietnam that the research team has sequenced.
The material used for the development of the diagnostic kit is an RNA sample extracted from SARS-Cov-2, which is responsible for Vietnamese patients provided by the National Institute of Hygiene and Epidemiology, genes as well as genomic regions cloning from SARS-Cov-2 RNA to serve as a model for research and provide sequences for the design of primers and probes. RNA samples of a human respiratory disease virus were provided by Central Military Hospital 108 and the Army Preventive Medicine Institute (Ministry of Defense).
PGS Quyen said that the kit published right after the new outbreak occurred based on the limited number of nCoV gene sequences, so the design primers sometimes could not be 100% specific, so the team collaborating with the National Institute of Hygiene and Epidemiology to transfer samples of important virus groups, develop Kit. After the gene sequences of the National Institute of Hygiene and Epidemiology, the team compared with the Covid-19 gene sequences in countries to select the most specific region to build corona virus in Vietnam.
Colonel Nguyen Phuc Thai, Deputy Director of the Institute of Military Preventive Medicine, said that the Institute participated in the initial evaluation of the biological kit developed by the Institute of Biotechnology. The Kit is eligible to apply Sars-Cov-2 diagnostics on clinical specimens, according to criteria of the Molecular Biological Virus Laboratory, Department of Biology, Army Preventive Medicine Institute.
SARS-Cov-2 Kit of the Institute of Biotechnology has the sensitivity and specificity equivalent to the World Health Organization's RT-PCR realtime Kit with 100% specificity, the sensitivity is 5 copies / reaction.
The study results confirmed that Vietnam has mastered modern technology and is completely active in creating the realtime RT-PCR Kit to diagnose SARS-Cov-2 in Vietnam, regardless of the foreign import.
"The next step is that the Military Preventive Medicine Institute will join the Institute of Biotechnology with a larger evaluation model, a larger number of samples for accurate results on the Kit. When results are available, they will be sent to the Covid-19 National Steering Committee director considered practical applications. 
Colonel Thai affirmed that the laboratory of the Institute had enough assessment capacity, standards and the amount of isolation in the army, so there were enough people to assess. He said he would continue to accompany and hope Kit will soon be put into application to solve the problem of screening a large number of patients in the country. However, he also said that with a successful research result but there are still many factors, it is unlikely to be put into application if it does not meet the speed, accuracy and cost.
Source: VNEXPRESS